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KMID : 0613820110210040502
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Journal of Life Science
2011 Volume.21 No. 4 p.502 ~ p.508
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Early Identification of Putative Zygotic Seedlings in Citrus Crosses between ‘Morita unshiu’ (Citrus. unshiu Marc.) and ‘Ponkan’ (C. reticulata Blanco) Using RAPD and SRAP
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Yun Su-Hyun
Moon Yong-Sun
Jin Seong-Beom
Kang In-Kyu
Lee Dong-Hoon
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Abstract
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The purpose of this study was to evaluate the methods of identifying zygotic seedlings of crosses between ‘Morita unshiu’ (Citrus. unshiuMarc.) and ‘Ponkan’ (C. reticulataBlanco). In order to investigate the frequency and position of zygotic seedlings and to determine the efficiency of zygotic seedling identification, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) were performed using UBC (9, 27, 229, 230, and 254) primers and F4/R27, F7/R14, F12/R10, and F44/R62 primer sets, respectively. A total of 37 putative zygotic seedlings out of 55 individuals were selected by RAPD and SRAP. The F7/R14 primer pair showed a screening ability of 45.5% (25/55), whereas the primer UBC27 revealed the highest efficiency of zygotic seedling identification (50.9%, 28/55). When both UBC27 and F7/R14 were properly used for selection of hybridized seedlings of ‘Morita unshiu’ (C. unshiuMarc.) and ‘Ponkan’ (C. reticulataBlanco), screening efficiency was increased to 60% (33/55) for putative zygotic seedlings. Thus, it is possible to select putative hybrid zygotic seedlings in an accurate and effective manner by RAPD and SRAP.
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KEYWORD
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DNA marker, hybrid zygotic seedling, polyembryony, polymorphic bands
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